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cd14 antibody, anti-human, reafinity  (Miltenyi Biotec)


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    Miltenyi Biotec cd14 antibody, anti-human, reafinity
    Cd14 Antibody, Anti Human, Reafinity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 256 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd14+apc/CD14+Antibody%2C+anti-human%2C+REAfinity/custom%40130-122-290%4042447860
    Average 95 stars, based on 256 article reviews
    cd14 antibody, anti-human, reafinity - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Expressing:

    Article Title: Optimization of hypo-alloimmunogenic multispecific CAR-T and SARS-CoV-2-specific T cells for off-the-shelf adoptive cell therapy
    Article Snippet: Cells acquisition and analysis was performed by BD LSRII and FlowJo, respectively (BD Bioscience). .. Antibodies to use: human CD45-APC (2D1; cat. 17-9459-42) and human HLA-ABC-PE (W6/32; cat. 12-9983-42) (both from eBioscience) for B2M KO; Biotin-CD19 CAR Detection Reagent (which recombinantly expressed fusion protein consisting of the human CD19 extracellular domains; cat. 130-115-965) and Streptavidin-APC-Vio 770 (cat. 130-106-794) for CAR expression; CD45-vioblue (5B1; cat. 130-113-122), CD3-FITC (REA613; cat. 130-113-138), CD4-viogreen (REA623; cat. 130-113-230), CD8-APC-vio770 (REA734; cat. 130-110-681), CD56-PE (REA196; cat. 130-113-312), CD14-APC (REA599; cat. 130-110-520), and CD19-PE-vio770 (REA675; cat. 130-113-647) for immune composition; CD3-FITC (REA613), CD45RO-APC-vio770 (REA611; cat. 130-113-557), and CD62L-PE (REA615; cat. 130-114-151) (all from Miltenyi Biotec) for T cell subsets of SARS-CoV-2-specific T cells; CD3-FITC, HLA-ABC-PE, and HLA-E (3D12; cat. 342606, Biolegend) for knockin analysis; CD3-BV421 (UCHT1; cat. 300434, BioLegend), CD56-FITC (REA196; cat. 130-113-312, Miltenyi Biotec), HLA-ABC-PE, and HLA-E for NK92MI co-culture; CD3-APC/Cyanine7 (SK7; cat. 344818, BioLegend), CD56-PE (REA196; cat. 130-114-549), NKG2A-FITC (REA110; cat. 130-113-565), and NKG2C-APC (REA205; cat. 130-117-547) (Miltenyi Biotec) for NK cells analysis. ..

    Article Title: A novel pseudotype derived of the canine distemper virus for adapter-mediated lentiviral transduction in vivo
    Article Snippet: .. Cellular composition of the PBMC prior to injection was determined using automated 553 analysis with the Express Mode template “CAR T cell immune composition” for the MACSQuantifyTM 554 software after staining with the respective antibody conjugates for CD45-VioBlue®, CD3-FITC, CD4-555 VioGreenTM, CD8-APCVio, CD14-APC, CD16-PE, CD56-PE (all Miltenyi Biotec, Bergisch Gladbach, 556 Germany) marker expression. ..

    Article Title: A novel pseudotype derived of the canine distemper virus for adapter-mediated lentiviral transduction in vivo
    Article Snippet: .. Cellular composition of the PBMCs prior to injection was determined using automated analysis with the Express Mode template “CAR T cell immune composition” for the MACSQuantify software after staining with the respective antibody conjugates for CD45-VioBlue, CD3-FITC, CD4-VioGreen, CD8-APCVio, CD14-APC, CD16-PE, and CD56-PE (all Miltenyi Biotec) marker expression. ..

    Knock-In:

    Article Title: Optimization of hypo-alloimmunogenic multispecific CAR-T and SARS-CoV-2-specific T cells for off-the-shelf adoptive cell therapy
    Article Snippet: Cells acquisition and analysis was performed by BD LSRII and FlowJo, respectively (BD Bioscience). .. Antibodies to use: human CD45-APC (2D1; cat. 17-9459-42) and human HLA-ABC-PE (W6/32; cat. 12-9983-42) (both from eBioscience) for B2M KO; Biotin-CD19 CAR Detection Reagent (which recombinantly expressed fusion protein consisting of the human CD19 extracellular domains; cat. 130-115-965) and Streptavidin-APC-Vio 770 (cat. 130-106-794) for CAR expression; CD45-vioblue (5B1; cat. 130-113-122), CD3-FITC (REA613; cat. 130-113-138), CD4-viogreen (REA623; cat. 130-113-230), CD8-APC-vio770 (REA734; cat. 130-110-681), CD56-PE (REA196; cat. 130-113-312), CD14-APC (REA599; cat. 130-110-520), and CD19-PE-vio770 (REA675; cat. 130-113-647) for immune composition; CD3-FITC (REA613), CD45RO-APC-vio770 (REA611; cat. 130-113-557), and CD62L-PE (REA615; cat. 130-114-151) (all from Miltenyi Biotec) for T cell subsets of SARS-CoV-2-specific T cells; CD3-FITC, HLA-ABC-PE, and HLA-E (3D12; cat. 342606, Biolegend) for knockin analysis; CD3-BV421 (UCHT1; cat. 300434, BioLegend), CD56-FITC (REA196; cat. 130-113-312, Miltenyi Biotec), HLA-ABC-PE, and HLA-E for NK92MI co-culture; CD3-APC/Cyanine7 (SK7; cat. 344818, BioLegend), CD56-PE (REA196; cat. 130-114-549), NKG2A-FITC (REA110; cat. 130-113-565), and NKG2C-APC (REA205; cat. 130-117-547) (Miltenyi Biotec) for NK cells analysis. ..

    Staining:

    Article Title: CRISPR-mediated generation of a tumor-associated antigen-deficient Raji platform to investigate antigen loss in CAR-T cell therapy
    Article Snippet: .. Staining was CD3-PE, CD4-FITC, CD8-FITC, CD14-APC, CD56-APC, CD19-APC, CD20-APC, CD22-APC (Miltenyi Biotec). ..

    Article Title: The DENGVAC study: Preliminary findings on the immunogenicity of the TAK-003 dengue vaccine.
    Article Snippet: Subsequently, the isolated PBMCs were resuspended in fetal bovine serum (Gibco Life Technologies) containing 10% dimethyl sulfoxide (DMSO) and cryopreserved in liquid nitrogen until use. .. Phenotypic staining The Monocytes (Mon) and Dendritic Cells (DC) profiles were evaluated using a cocktail of monoclonal antibodies (MAbs) containing the following conjugated MAbs: CD3 Krome Orange, CD14 APC, CD16 PCY7, 7AAD, CD80 APC750, CD86 V450 for monocytes staining; LIN-1 FITC, CD123 PE, CD11c PCC7, HLA-DR ECD, CD80 APC750, CD86 V450 for DC staining (BD Biosciences, Beckman Coulter and Miltenyi). .. Briefly, 100 μL of whole blood suspension was loaded into the DuraClone tube and incubated at room temperature for 15 min. VersaLyse Lysing Solution (Beckman Coulter) was added, and the mixture was incubated for 15 min. Then, samples were washed twice with phosphate-buffered saline (PBS) and fixed with paraformaldehyde (PFA, Sigma).

    Article Title: Reducing CD33 expression to selectively protect therapeutic cells
    Article Snippet: .. Flow cytometry staining was performed with human CD45-PerCP (Clone 2D1), mouse CD45.1/CD45.2-V500 (Clone 30-F11), CD3-FITC or -APC (Clone UCHT1), CD4-V450 (Clone RPA-T4), CD20-PE (Clone 2H7), CD14-APC or -PE-Cy7 (Clone M5E2), CD34-APC (Clone 581) (all from BD Biosciences, San Jose, CA), and CD33-PE (Clone AC104.3E3, Miltenyi Biotec). ..

    Article Title: A novel pseudotype derived of the canine distemper virus for adapter-mediated lentiviral transduction in vivo
    Article Snippet: .. Cellular composition of the PBMC prior to injection was determined using automated 553 analysis with the Express Mode template “CAR T cell immune composition” for the MACSQuantifyTM 554 software after staining with the respective antibody conjugates for CD45-VioBlue®, CD3-FITC, CD4-555 VioGreenTM, CD8-APCVio, CD14-APC, CD16-PE, CD56-PE (all Miltenyi Biotec, Bergisch Gladbach, 556 Germany) marker expression. ..

    Article Title: A novel pseudotype derived of the canine distemper virus for adapter-mediated lentiviral transduction in vivo
    Article Snippet: .. Cellular composition of the PBMCs prior to injection was determined using automated analysis with the Express Mode template “CAR T cell immune composition” for the MACSQuantify software after staining with the respective antibody conjugates for CD45-VioBlue, CD3-FITC, CD4-VioGreen, CD8-APCVio, CD14-APC, CD16-PE, and CD56-PE (all Miltenyi Biotec) marker expression. ..

    Bioprocessing:

    Article Title: The DENGVAC study: Preliminary findings on the immunogenicity of the TAK-003 dengue vaccine.
    Article Snippet: Subsequently, the isolated PBMCs were resuspended in fetal bovine serum (Gibco Life Technologies) containing 10% dimethyl sulfoxide (DMSO) and cryopreserved in liquid nitrogen until use. .. Phenotypic staining The Monocytes (Mon) and Dendritic Cells (DC) profiles were evaluated using a cocktail of monoclonal antibodies (MAbs) containing the following conjugated MAbs: CD3 Krome Orange, CD14 APC, CD16 PCY7, 7AAD, CD80 APC750, CD86 V450 for monocytes staining; LIN-1 FITC, CD123 PE, CD11c PCC7, HLA-DR ECD, CD80 APC750, CD86 V450 for DC staining (BD Biosciences, Beckman Coulter and Miltenyi). .. Briefly, 100 μL of whole blood suspension was loaded into the DuraClone tube and incubated at room temperature for 15 min. VersaLyse Lysing Solution (Beckman Coulter) was added, and the mixture was incubated for 15 min. Then, samples were washed twice with phosphate-buffered saline (PBS) and fixed with paraformaldehyde (PFA, Sigma).

    Incubation:

    Article Title: Effects of Human Recombinant Growth Hormone (rhGH) Treatment on Plasma Extracellular Vesicles in GH-Deficient Children: A Preliminary Report
    Article Snippet: .. Each aliquot of CFSE-stained sample was incubated with a specific antibody: CD14-APC (clone TÜK4) for monocyte/macrophage-derived EVs, CD62E (clone REA280) for endothelium-derived EVs, CD42a-APC (clone REA209) for platelet-derived EVs (Miltenyi Biotec, Bergisch Gladbach, Germany), A-FABP (clone B-4) for adipocyte-derived EVs, and α-sarcoglycan SCGA (clone F-7) for skeletal muscle-derived EVs (Santa Cruz Biotechnology, Dallas, TX, USA). ..

    Article Title: Effects of Human Recombinant Growth Hormone (rhGH) Treatment on Plasma Extracellular Vesicles in GH-Deficient Children: A Preliminary Report.
    Article Snippet: .. Each aliquot of CFSEstained sample was incubated with a specific antibody: CD14-APC (clone TÜK4) for monocyte/macrophage-derived EVs, CD62E (clone REA280) for endothelium-derived EVs, CD42a-APC (clone REA209) for platelet-derived EVs (Miltenyi Biotec, Bergisch Gladbach, Germany), A-FABP (clone B-4) for adipocyte-derived EVs, and α-sarcoglycan SCGA (clone F-7) for skeletal muscle-derived EVs (Santa Cruz Biotechnology, Dallas, TX, USA). ..

    Flow Cytometry:

    Article Title: Reducing CD33 expression to selectively protect therapeutic cells
    Article Snippet: .. Flow cytometry staining was performed with human CD45-PerCP (Clone 2D1), mouse CD45.1/CD45.2-V500 (Clone 30-F11), CD3-FITC or -APC (Clone UCHT1), CD4-V450 (Clone RPA-T4), CD20-PE (Clone 2H7), CD14-APC or -PE-Cy7 (Clone M5E2), CD34-APC (Clone 581) (all from BD Biosciences, San Jose, CA), and CD33-PE (Clone AC104.3E3, Miltenyi Biotec). ..

    Recombinase Polymerase Amplification:

    Article Title: Reducing CD33 expression to selectively protect therapeutic cells
    Article Snippet: .. Flow cytometry staining was performed with human CD45-PerCP (Clone 2D1), mouse CD45.1/CD45.2-V500 (Clone 30-F11), CD3-FITC or -APC (Clone UCHT1), CD4-V450 (Clone RPA-T4), CD20-PE (Clone 2H7), CD14-APC or -PE-Cy7 (Clone M5E2), CD34-APC (Clone 581) (all from BD Biosciences, San Jose, CA), and CD33-PE (Clone AC104.3E3, Miltenyi Biotec). ..

    Injection:

    Article Title: A novel pseudotype derived of the canine distemper virus for adapter-mediated lentiviral transduction in vivo
    Article Snippet: .. Cellular composition of the PBMC prior to injection was determined using automated 553 analysis with the Express Mode template “CAR T cell immune composition” for the MACSQuantifyTM 554 software after staining with the respective antibody conjugates for CD45-VioBlue®, CD3-FITC, CD4-555 VioGreenTM, CD8-APCVio, CD14-APC, CD16-PE, CD56-PE (all Miltenyi Biotec, Bergisch Gladbach, 556 Germany) marker expression. ..

    Article Title: A novel pseudotype derived of the canine distemper virus for adapter-mediated lentiviral transduction in vivo
    Article Snippet: .. Cellular composition of the PBMCs prior to injection was determined using automated analysis with the Express Mode template “CAR T cell immune composition” for the MACSQuantify software after staining with the respective antibody conjugates for CD45-VioBlue, CD3-FITC, CD4-VioGreen, CD8-APCVio, CD14-APC, CD16-PE, and CD56-PE (all Miltenyi Biotec) marker expression. ..

    Software:

    Article Title: A novel pseudotype derived of the canine distemper virus for adapter-mediated lentiviral transduction in vivo
    Article Snippet: .. Cellular composition of the PBMC prior to injection was determined using automated 553 analysis with the Express Mode template “CAR T cell immune composition” for the MACSQuantifyTM 554 software after staining with the respective antibody conjugates for CD45-VioBlue®, CD3-FITC, CD4-555 VioGreenTM, CD8-APCVio, CD14-APC, CD16-PE, CD56-PE (all Miltenyi Biotec, Bergisch Gladbach, 556 Germany) marker expression. ..

    Article Title: A novel pseudotype derived of the canine distemper virus for adapter-mediated lentiviral transduction in vivo
    Article Snippet: .. Cellular composition of the PBMCs prior to injection was determined using automated analysis with the Express Mode template “CAR T cell immune composition” for the MACSQuantify software after staining with the respective antibody conjugates for CD45-VioBlue, CD3-FITC, CD4-VioGreen, CD8-APCVio, CD14-APC, CD16-PE, and CD56-PE (all Miltenyi Biotec) marker expression. ..

    Marker:

    Article Title: A novel pseudotype derived of the canine distemper virus for adapter-mediated lentiviral transduction in vivo
    Article Snippet: .. Cellular composition of the PBMC prior to injection was determined using automated 553 analysis with the Express Mode template “CAR T cell immune composition” for the MACSQuantifyTM 554 software after staining with the respective antibody conjugates for CD45-VioBlue®, CD3-FITC, CD4-555 VioGreenTM, CD8-APCVio, CD14-APC, CD16-PE, CD56-PE (all Miltenyi Biotec, Bergisch Gladbach, 556 Germany) marker expression. ..

    Article Title: A novel pseudotype derived of the canine distemper virus for adapter-mediated lentiviral transduction in vivo
    Article Snippet: .. Cellular composition of the PBMCs prior to injection was determined using automated analysis with the Express Mode template “CAR T cell immune composition” for the MACSQuantify software after staining with the respective antibody conjugates for CD45-VioBlue, CD3-FITC, CD4-VioGreen, CD8-APCVio, CD14-APC, CD16-PE, and CD56-PE (all Miltenyi Biotec) marker expression. ..



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